RNA-based stable isotope probing and isolation of anaerobic benzene-degrading bacteria from gasoline-contaminated groundwater.

@article{Kasai2006RNAbasedSI,
  title={RNA-based stable isotope probing and isolation of anaerobic benzene-degrading bacteria from gasoline-contaminated groundwater.},
  author={Yuki Kasai and Y. Takahata and Mike Manefield and Kazuya Watanabe},
  journal={Applied and environmental microbiology},
  year={2006},
  volume={72 5},
  pages={3586-92}
}
Stable isotope probing (SIP) of benzene-degrading bacteria in gasoline-contaminated groundwater was coupled to denaturing gradient gel electrophoresis (DGGE) of DNA fragments amplified by reverse transcription-PCR from community 16S rRNA molecules. Supplementation of the groundwater with [(13)C(6)]benzene together with an electron acceptor (nitrate, sulfate, or oxygen) showed that a phylotype affiliated with the genus Azoarcus specifically appeared in the (13)C-RNA fraction only when nitrate… CONTINUE READING
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Assessment of chemical and microbiological signatures during natural attenuation of gasoline-contaminated groundwater, p. 827–831

  • Y. Takahata, Y. Kasai, K. Watanabe
  • In Proceedings of the European Symposium on…
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