RNA at 92°C

  title={RNA at 92°C},
  author={Claire Toffano-Nioche and Alban Ott and Estelle Crozat and An N. Nguyen and Matthias Zytnicki and Fabrice Leclerc and Patrick Forterre and Philippe Bouloc and Daniel Gautheret},
  booktitle={RNA biology},
The non-coding transcriptome of the hyperthermophilic archaeon Pyrococcus abyssi is investigated using the RNA-seq technology. A dedicated computational pipeline analyzes RNA-seq reads and prior genome annotation to identify small RNAs, untranslated regions of mRNAs, and cis-encoded antisense transcripts. Unlike other archaea, such as Sulfolobus and Halobacteriales, P. abyssi produces few leaderless mRNA transcripts. Antisense transcription is widespread (215 transcripts) and targets protein… CONTINUE READING