RNA aptamers and spiegelmers: synthesis, purification, and post-synthetic PEG conjugation.


This unit describes the solid-phase synthesis and downstream processing for RNA oligonucleotides with a length of up to 40 to 50 nucleotides on a 1- to 4-mmol scale with subsequent conjugation to PEG using the L-RNA spiegelmer NOX-E36 as an example. Following synthesis and two-step deprotection, the crude oligonucleotide is purified by preparative reversed… (More)
DOI: 10.1002/0471142700.nc0446s46


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