RNA analysis by biosynthetic tagging using 4-thiouracil and uracil phosphoribosyltransferase.

@article{Zeiner2008RNAAB,
  title={RNA analysis by biosynthetic tagging using 4-thiouracil and uracil phosphoribosyltransferase.},
  author={Gusti M. Zeiner and Michael D. Cleary and Ashley E Fouts and Christopher D Meiring and Edward S Mocarski and John C. Boothroyd},
  journal={Methods in molecular biology},
  year={2008},
  volume={419},
  pages={
          135-46
        }
}
RNA analysis by biosynthetic tagging (RABT) enables sensitive and specific queries of (a) how gene expression is regulated on a genome-wide scale and (b) transcriptional profiling of a single cell or tissue type in vivo. RABT can be achieved by exploiting unique properties of Toxoplasma gondii uracil phosphoribosyltransferase (TgUPRT), a pyrimidine salvage enzyme that couples ribose-5-phosphate to the N1 nitrogen of uracil to yield uridine monophosphate (UMP). When 4-thiouracil is provided as a… 
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