RNA activation in insects: the targeted activation of endogenous and exogenous genes.

  title={RNA activation in insects: the targeted activation of endogenous and exogenous genes.},
  author={Lachlan De Hayr and Sultan Asad and M. Raffiq Hussain and Sassan Asgari},
  journal={Insect biochemistry and molecular biology},



RNA interference technology in crop protection against arthropod pests, pathogens and nematodes.

This review goes beyond the classical presentation of successful examples of RNAi in pest-insect control and comprehensively explore its potential for the control of plant pathogens, nematodes and mites, and to fight against diseases and parasites in beneficial insects.

RNAi mechanisms and applications.

Current knowledge about the mechanisms and applications of RNAi, a compendium of mechanisms involving small RNAs that regulate the expression of genes in a variety of eukaryotic organisms, are summarized.

Small RNA and transcriptional upregulation

Given the potential of RNAa as a molecular tool for studying gene function and as a therapeutic for disease, further research is needed to completely elucidate its molecular mechanism in order to refine the rules for target selection and improve strategies for exploiting it therapeutically.

Inducing gene expression by targeting promoter sequences using small activating RNAs.

A detailed protocol for target selection and dsRNA design with associated experiments to facilitate RNAa in cultured cells is described and may be applied to selectively activate endogenous gene expression for studying gene function, interrogating molecular pathways and reprogramming cell fate.

Small dsRNAs induce transcriptional activation in human cells

A more diverse role for small RNA molecules in the regulation of gene expression than previously recognized is revealed and a potential therapeutic use for dsRNA in targeted gene activation is identified.

Small activating RNA binds to the genomic target site in a seed-region-dependent manner

It is demonstrated that saRNAs work via an on-site mechanism by binding to target genomic DNA in a seed-region-dependent manner, reminiscent of miRNA-like target recognition.

RNA activation technique and its applications in cancer research.

This paper reviews the literature on RNAa with regard to the course of discovery, the mechanisms and characteristics of action, and the current status and prospects of application.

RNAa Is Conserved in Mammalian Cells

This study demonstrates that RNAa exists in mammalian species other than human, and suggests that nonhuman primate disease models may have clinical applicability for validating RNAa-based drugs.

Design and Experimental Validation of Small Activating RNAs Targeting an Exogenous Promoter in Human Cells.

The design and experimental investigation of the first exogenous genetic construct to be successfully targeted by RNA activation, a phenomenon whereby small double-stranded RNAs increase gene expression from sequence-similar promoters by a mechanism thought to be related to that of RNA interference.