RNA Structure: Reading the Ribosome

@article{Noller2005RNASR,
  title={RNA Structure: Reading the Ribosome},
  author={Harry F. Noller},
  journal={Science},
  year={2005},
  volume={309},
  pages={1508 - 1514}
}
  • H. Noller
  • Published 2 September 2005
  • Biology, Chemistry
  • Science
The crystal structures of the ribosome and its subunits have increased the amount of information about RNA structure by about two orders of magnitude. This is leading to an understanding of the principles of RNA folding and of the molecular interactions that underlie the functional capabilities of the ribosome and other RNA systems. Nearly all of the possible types of RNA tertiary interactions have been found in ribosomal RNA. One of these, an abundant tertiary structural motif called the A… 
View on AAAS
psi.toronto.edu
320 Citations
Highly Influential Citations
9
Background Citations
111
Methods Citations
7
Results Citations
1

320 Citations

G-ribo: a new structural motif in ribosomal RNA.

Analysis of the available crystal structures of the ribosome and of its subunits has revealed a new RNA motif that we call G-ribo. The motif consists of two double helices positioned side-by-side and…

RNA recurrent motifs : identification and characterization

Systematic analysis of the ribosome as well as the RNAseP crystal structures allowed for the identification of a new RNA motif, which is called “DTJ”, or DoubleTwist Joint motif, made of three short RNA double helices, which stack one on top of another.

Correlation of the structure and conformational changes of selected fragments of plant small ribosomal RNA within the steps of polypeptide chain elongation.

RNA Structures and Their Diversity

Mutational analysis of the ribosome.

Editing Reactions from the Perspective of RNA Structure

The focus of this review is to highlight the contribu- tions of RNA structure to the specificity and efficiency of RNA editing.

The structure and function of catalytic RNAs

The structure-function relationship of both small and large ribozymes is reviewed, especially the structural basis of their catalysis.

Assembly of bacterial ribosomes.

The history of the early work on ribosome assembly in Escherichia coli is presented, including a description of in vivo and in vitro intermediates, and the effects of ribosomal proteins in driving these events are explored.

Complexes of tRNA and maturation enzymes: shaping up for translation.

  • Hong Li
  • Biology, Chemistry
    Current opinion in structural biology
  • 2007

Interactions between terminal ribosomal RNA helices stabilize the Escherichia coli large ribosomal subunit

Cryo-EM images show that the loss of RNA-RNA tertiary contacts that bridge helices H1, H94, and H98 as the cause of destabilization is identified and can be restored through the insertion of a single adenosine in the extended H98 helix.
...

94 References

Structure of the 30S ribosomal subunit

The crystal structure of the 30S subunit from Thermus thermophilus, refined to 3 Å resolution, is reported, which will facilitate the interpretation in molecular terms of lower resolution structural data on several functional states of the ribosome from electron microscopy and crystallography.

Structure of the 70 S ribosome: implications for movement.

Compared the X-ray crystal structures of three different functional states of the 30 S ribosomal subunit of the Ramakrishnan group, finding many conformational differences found, distributed over the whole structure.

The structural basis of large ribosomal subunit function.

The work reviewed proves beyond doubt that the ribosome is an RNA enzyme, as had long been surmised on the basis of less conclusive evidence.

X-ray crystal structures of 70S ribosome functional complexes.

Structures of 70S ribosome complexes containing messenger RNA and transfer RNA (tRNA), or tRNA analogs, have been solved by x-ray crystallography at up to 7.8 angstrom resolution. Many details of the…

RNA-protein interactions in 30S ribosomal subunits: folding and function of 16S rRNA.

Several lines of evidence suggest that protein-dependent conformational changes in 16S rRNA play an important part in the cooperativity of ribosome assembly and in fine-tuning of the conformation and dynamics of 16SrRNA in the 30S subunit.

Crystal structure of an initiation factor bound to the 30S ribosomal subunit.

The crystal structure of a complex of IF1 and the 30S ribosomal subunit is reported, explaining how localized changes at the ribosome A site lead to global alterations in the conformation of the30S subunit.

Crystal Structure of the Ribosome at 5.5 Å Resolution

The crystal structure of the complete Thermus thermophilus 70S ribosome containing bound messenger RNA and transfer RNAs (tRNAs) at 5.5 angstrom resolution is described, suggesting coupling of the 20 to 50 angstrom movements associated with tRNA translocation with intersubunit movement.

5S RNA secondary structure

It is possible that functional conformations of the 5SRNA molecule need only exist in the context of the ribosome, and consequently, may only exist therein, and the interpretation of experimental investigations on the isolated 5S RNA molecule may have inherent difficulties.

The structure of ribosomal RNA and its organization relative to ribosomal protein.

The roles of ribosomal proteins in the structure assembly, and evolution of the large ribosomal subunit.

...