RNA Sequencing and Analysis.

@article{Kukurba2015RNASA,
  title={RNA Sequencing and Analysis.},
  author={Kimberly R. Kukurba and Stephen B. Montgomery},
  journal={Cold Spring Harbor protocols},
  year={2015},
  volume={2015 11},
  pages={
          951-69
        }
}
RNA sequencing (RNA-Seq) uses the capabilities of high-throughput sequencing methods to provide insight into the transcriptome of a cell. Compared to previous Sanger sequencing- and microarray-based methods, RNA-Seq provides far higher coverage and greater resolution of the dynamic nature of the transcriptome. Beyond quantifying gene expression, the data generated by RNA-Seq facilitate the discovery of novel transcripts, identification of alternatively spliced genes, and detection of allele… CONTINUE READING

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