RNA-Seq Reveals Spliceosome and Proteasome Genes as Most Consistent Transcripts in Human Cancer Cells

@inproceedings{Macrae2013RNASeqRS,
  title={RNA-Seq Reveals Spliceosome and Proteasome Genes as Most Consistent Transcripts in Human Cancer Cells},
  author={Tara Macrae and Tobias J. Sargeant and S{\'e}bastien Lemieux and J. -L. Hebert and {\'E}ric Deneault and Guy Sauvageau},
  booktitle={PloS one},
  year={2013}
}
Accurate quantification of gene expression by qRT-PCR relies on normalization against a consistently expressed control gene. However, control genes in common use often vary greatly between samples, especially in cancer. The advent of Next Generation Sequencing technology offers the possibility to better select control genes with the least cell to cell variability in steady state transcript levels. Here we analyze the transcriptomes of 55 leukemia samples to identify the most consistent genes… CONTINUE READING