RNA-Seq Analysis of Differentiated Keratinocytes Reveals a Massive Response to Late Events during Human Papillomavirus 16 Infection, Including Loss of Epithelial Barrier Function

@inproceedings{Klymenko2017RNASeqAO,
  title={RNA-Seq Analysis of Differentiated Keratinocytes Reveals a Massive Response to Late Events during Human Papillomavirus 16 Infection, Including Loss of Epithelial Barrier Function},
  author={Tetyana Klymenko and Quan Xin Gu and I. Herbert and Andrew Stevenson and Valentin Vankov Iliev and Gemma L Watkins and Catherine E Pollock and Ramya Bhatia and Kate Cuschieri and Pawel Herzyk and Derek Gatherer and Sheila V Graham},
  booktitle={Journal of virology},
  year={2017}
}
The human papillomavirus (HPV) replication cycle is tightly linked to epithelial cell differentiation. To examine HPV-associated changes in the keratinocyte transcriptome, RNAs isolated from undifferentiated and differentiated cell populations of normal, spontaneously immortalised, keratinocytes (NIKS), and NIKS stably transfected with HPV16 episomal genomes (NIKS16), were compared using RNASeq. HPV16 infection altered expression of 2862 cellular genes. Next, to elucidate the role of… CONTINUE READING