RNA Mimics of Green Fluorescent Protein

  title={RNA Mimics of Green Fluorescent Protein},
  author={Samie R. Jaffrey},
  pages={642 - 646}
  • S. Jaffrey
  • Published 29 July 2011
  • Biology, Chemistry
  • Science
Selection for RNAs that bind and activate derivatives of the green fluorescent protein fluorophore yields a wide range of useful spectral properties. Green fluorescent protein (GFP) and its derivatives have transformed the use and analysis of proteins for diverse applications. Like proteins, RNA has complex roles in cellular function and is increasingly used for various applications, but a comparable approach for fluorescently tagging RNA is lacking. Here, we describe the generation of RNA… 
RNA imaging in bacteria.
The main single-cell techniques for RNA visualization with fluorescence microscopy are reviewed, and their applications in bacteria are described.
Development of a riboswitch-based platform for live cell imaging of RNAs in mammalian cells
An RNA imaging platform using the Cobalamin (Cbl) riboswitch as an RNA aptamer and a series of probes containing Cbl as a fluorescent quencher linked to a range of fluorophores is developed.
RNA modularity for synthetic biology
A better understanding and integration of three-dimensional RNA modularity at structural and functional levels is critical to the development of more complex, functional bio-systems and molecular machines for synthetic biology applications.
RNA complex purification using high‐affinity fluorescent RNA aptamer tags
This work compares and contrast existing RNA‐ and protein‐purification strategies with the potential of new RNA‐tagging systems such as RNA Spinach and RNA Mango, and suggests derivatized dyes can be used in conjunction with these aptamers to purify RNA complexes while simultaneously using their intrinsic fluorescence to track the complex of interest.
A turn-on aptamer for fluorescent labeling of RNA
This review of recent developments in the field of genetically encoded probes for RNA imaging highlights recent developments and discusses the strengths and limitations of the different approaches.
Current techniques for visualizing RNA in cells
This review aims to outline the current state-of-the-art techniques and point out their benefits and limitations.
Live Cell Imaging of Endogenous mRNA Using RNA-Based Fluorescence "Turn-On" Probe.
RNA-based fluorescence "turn-on" probes that target mRNA by fusing an unstable form of Spinach with target-complementary sequences are designed and demonstrated to be selective, stable, and capable of targeting various mRNAs for live E. coli imaging.
Techniques for RNA in vivo imaging in plants
The currently available techniques for plant RNA in vivo imaging are introduced and their suitability for different biological questions are discussed.
Designing optogenetically controlled RNA for regulating biological systems
The challenges of and potential strategies for engineering light regulation into functional RNAs in cells are explored and applications of light‐regulated RNAs for synthetic biology and for studying functions of endogenously expressed RNAs are discussed.
Live-cell imaging of mammalian RNAs with Spinach2.


Methods for measurement of intracellular magnesium: NMR and fluorescence.
  • R. London
  • Chemistry
    Annual review of physiology
  • 1991
This review covers two methods, in vivo nuclear magnetic resonance (NMR) and fluorescence spectroscopy, which have provided useful means for the determinations based on ATP measurements, and more recently developed fluorinated or fluorescent indicators.
Reversible denaturation of Aequorea green-fluorescent protein: physical separation and characterization of the renatured protein.
The green-fluorescent protein (GFP) that functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea has been renatured with up to 90% yield following acid, base, or guanidine