RNA Isolation method for polysaccharide rich algae: agar producing Gracilaria tenuistipitata (Rhodophyta)

@article{Falco2007RNAIM,
  title={RNA Isolation method for polysaccharide rich algae: agar producing Gracilaria tenuistipitata (Rhodophyta)},
  author={Vanessa dos Reis Falc{\~a}o and Angela Pedroso Tonon and Mariana Cabral de Oliveira and Pio Colepicolo},
  journal={Journal of Applied Phycology},
  year={2007},
  volume={20},
  pages={9-12}
}
RNA isolation is essential to study gene expression at the molecular level. However, RNA isolation is difficult in organisms (plants and algae) that contain large amounts of polysaccharides, which co-precipitate with RNA. Currently, there is no commercial kit available, specifically for the isolation of high-quality RNA from these organisms. Furthermore, because of the large amounts of polysaccharides, the common protocols for RNA isolation usually result in poor yields when applied to algae… CONTINUE READING