RIP-chip-SRM--a new combinatorial large-scale approach identifies a set of translationally regulated bantam/miR-58 targets in C. elegans.

@article{Jovanovic2012RIPchipSRMaNC,
  title={RIP-chip-SRM--a new combinatorial large-scale approach identifies a set of translationally regulated bantam/miR-58 targets in C. elegans.},
  author={Marko Jovanovic and Lukas Reiter and Alejandra M. Clark and Manuel Weiss and Paola Picotti and Hubert Rehrauer and Andreas Frei and Lukas J Neukomm and Ethan Kaufman and Bernd Wollscheid and Martin J Simard and Eric A Miska and Ruedi Aebersold and Andr{\'e} P Gerber and Michael O. Hengartner},
  journal={Genome research},
  year={2012},
  volume={22 7},
  pages={1360-71}
}
MicroRNAs (miRNAs) are small, noncoding RNAs that negatively regulate gene expression. As miRNAs are involved in a wide range of biological processes and diseases, much effort has been invested in identifying their mRNA targets. Here, we present a novel combinatorial approach, RIP-chip-SRM (RNA-binding protein immunopurification + microarray + targeted protein quantification via selected reaction monitoring), to identify de novo high-confidence miRNA targets in the nematode Caenorhabditis… CONTINUE READING