RIP-chip-SRM--a new combinatorial large-scale approach identifies a set of translationally regulated bantam/miR-58 targets in C. elegans.

@article{Jovanovic2012RIPchipSRMaNC,
  title={RIP-chip-SRM--a new combinatorial large-scale approach identifies a set of translationally regulated bantam/miR-58 targets in C. elegans.},
  author={Marko Jovanovic and Lukas Reiter and Alejandra Clark and Manuel Weiss and Paola Picotti and Hubert Rehrauer and Andreas Frei and Lukas J. Neukomm and Ethan Kaufman and Bernd Wollscheid and Martin J Simard and Eric A. Miska and Ruedi Aebersold and Andr{\'e} P Gerber and Michael O. Hengartner},
  journal={Genome research},
  year={2012},
  volume={22 7},
  pages={
          1360-71
        }
}
  • Marko Jovanovic, Lukas Reiter, +12 authors Michael O. Hengartner
  • Published 2012
  • Medicine, Biology
  • Genome research
  • MicroRNAs (miRNAs) are small, noncoding RNAs that negatively regulate gene expression. As miRNAs are involved in a wide range of biological processes and diseases, much effort has been invested in identifying their mRNA targets. Here, we present a novel combinatorial approach, RIP-chip-SRM (RNA-binding protein immunopurification + microarray + targeted protein quantification via selected reaction monitoring), to identify de novo high-confidence miRNA targets in the nematode Caenorhabditis… CONTINUE READING

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