RH70, a bidirectional RNA helicase, co-purifies with U1snRNP.

  title={RH70, a bidirectional RNA helicase, co-purifies with U1snRNP.},
  author={Chee Gun Lee},
  journal={The Journal of biological chemistry},
  volume={277 42},
  • Chee Gun Lee
  • Published 2002 in The Journal of biological chemistry
In this report, I describe the co-purification of a novel 70-kDa RNA helicase (RH70) and U1snRNP through six column steps. Peptide sequence analysis by mass spectrometry and Edman degradation revealed that RH70 is the previously reported DDX17. Biochemical characterization of RH70, obtained by partial separation from U1snRNP, yielded the following results. (a) RH70 mediates the unwinding of duplex RNA but not DNA in an ATP-dependent manner. (b) Both the RNA-dependent ATPase and RNA helicase… CONTINUE READING