RGD-tethered Silk Substrate Stimulates the Differentiation of Human Tendon Cells

  title={RGD-tethered Silk Substrate Stimulates the Differentiation of Human Tendon Cells},
  author={Tarik Kardestuncer and Mary Beth Mccarthy and Vassilis Karageorgiou and David L. Kaplan and Gloria A. Gronowicz},
  journal={Clinical Orthopaedics and Related Research},
Tendon reconstruction surgery often requires healing of the tendon to bone. The development of a more rapid and strong interaction at the tendon to bone interface would be invaluable to patients having orthopaedic surgery. Therefore, our rationale was to modify sutures so that they would be anabolic for tendon to bone healing. It has been shown that silk stimulates bone formation in osteoblast cultures. In the current study, we tested the ability of silk and silk-RGD (arginine-glycine-aspartic… 

Silk as a Biomaterial.

Biologic enhancement of a common arthroscopic suture.

Tendon Tissue Engineering: Cells, Growth Factors, Scaffolds and Production Techniques.

Characterization of tendon cell cultures of the human rotator cuff.

This study represents a characterization of tenocyte-like cells from the human rotator cuff as close as possible and allows further studies to improve production of tendon matrix and osteofibroblastic integration at the tendon-bone unit following tendon repair.

Albumin-Coated Bioactive Suture for Cell Transplantation

Coating absorbable suture with proteins, especially serum albumin, improves attachment and proliferation of cells, and only 48 hours in culture is enough to cover the sutures sufficiently, resulting in short-term and long-term survival of cells.

Mesenchymal stem cell-seeded multilayered dense collagen-silk fibroin hybrid for tissue engineering applications.

The ease of multilayered construct fabrication, enhanced biomechanical properties, along with uniformity of cell distribution confirmed the possibility for the incorporation and segregation of different cell types within distinct layers for the regeneration of complex tissues, such as skin, or central nervous system dura mater.



Functionalized silk-based biomaterials for bone formation.

RGD covalently decorated silk appears to stimulate osteoblast-based mineralization in vitro and indicates that the proteins serve as suitable bone-inducing matrices.

Use of Recombinant Human Bone Morphogenetic Protein-2 to Enhance Tendon Healing in a Bone Tunnel

It is demonstrated that bone morphogenetic protein can accelerate the healing process when a tendon graft is transplanted into a bone tunnel and suggested superior healing at the lower protein dose.

Human bone marrow stromal cell and ligament fibroblast responses on RGD-modified silk fibers.

The ability of RGD-coupled silk matrices to support BMSC attachment, which leads to higher cell density and collagen matrix production in vitro, combined with mechanical, fatigue, and biocompatibility properties of the silk protein matrix, suggest potential for use of this biomaterial for tissue engineering.

Engineering bone-like tissue in vitro using human bone marrow stem cells and silk scaffolds.

The results suggest that RGD-silk scaffolds are particularly suitable for autologous bone tissue engineering, presumably because of their stable macroporous structure, tailorable mechanical properties matching those of native bone, and slow degradation.

Use of mesenchymal stem cells in a collagen matrix for achilles tendon repair

The results indicate that delivering mesenchymal stem cell‐contracted, organized collagen implants to large tendon defects can significantly improve the biomechanics, structure, and probably the function of the tendon after injury.

Enhancement of tendon graft osteointegration using mesenchymal stem cells in a rabbit model of anterior cruciate ligament reconstruction.

  • J. LimJ. HuiLi LiA. ThambyahJ. GohE. Lee
  • Biology, Medicine
    Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association
  • 2004

Bone morphogenetic protein-2 decorated silk fibroin films induce osteogenic differentiation of human bone marrow stromal cells.

The results illustrate that BMP-2 covalently coupled on silk biomaterial matrices retains biological function in vitro based on the induction of osteogenic markers in seeded bone marrow stromal cells.

Augmentation of Tendon Healing in an Intraarticular Bone Tunnel with Use of a Bone Growth Factor *

The potential for augmenting tendon healing in an intraarticular bone tunnel using an osteoinductive growth factor is demonstrated using a rabbit anterior cruciate ligament-reconstruction model.

Attachment and spreading of fibroblasts on an RGD peptide-modified injectable hyaluronan hydrogel.

Attachment, spreading, and proliferation of cells is dramatically enhanced on RGD-modified surfaces but only modestly accelerated in vivo tissue formation.

Tendon-healing in a bone tunnel. A biomechanical and histological study in the dog.

The progressive increase in strength was correlated with the degree of bone ingrowth, mineralization, and maturation of the healing tissue, noted histologically.