RGB marking with lentiviral vectors for multicolor clonal cell tracking

@article{Weber2012RGBMW,
  title={RGB marking with lentiviral vectors for multicolor clonal cell tracking},
  author={Kristoffer Weber and Michael Thomaschewski and Daniel Benten and Boris Fehse},
  journal={Nature Protocols},
  year={2012},
  volume={7},
  pages={839-849}
}
Cells transduced with lentiviral vectors are individually marked by a highly characteristic pattern of insertion sites inherited by all their progeny. We have recently extended this principle of clonal cell marking by introducing the method of RGB marking, which makes use of the simultaneous transduction of target cells with three lentiviral gene ontology (LeGO) vectors encoding red, green or blue fluorescent proteins. In accordance with the additive color model, individual RGB-marked cells… CONTINUE READING
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