RFLP Evaluation of Nine Medicago Accessions Representing the Original Germplasm Sources for North American Alfalfa Cultivars

  title={RFLP Evaluation of Nine Medicago Accessions Representing the Original Germplasm Sources for North American Alfalfa Cultivars},
  author={Kimberlee K. Kidwell and Daniel F. C. Austin and Thomas C. Osborn},
  journal={Crop Science},
All alfalfa (Medicago sativa L.) cultivars grown in North America originate from nine germplasm sources introduced into the USA from different geographic regions. Nine accessions representing the original germplasm sources have been developed; however, the level of genetic diversity within and among these accessions is not known. Genetic diversity of these accessions was estimated by screening DNAs from 12 individuals of each accession, along with bulked samples containing equal amounts of DNA… 

Estimating genetic relationships among historical sources of alfalfa germplasm and selected cultivars with sequence related amplified polymorphisms

It is demonstrated that SRAPs are a promising marker system for detecting polymorphisms in alfalfa using sequence related amplified polymorphisms (SRAPs) and that relationships based on SRAP analysis appear to generally concur with expected relationshipsbased on fall dormancy.

Non random chloroplast DNA hypervariability in Medicago sativa

  • D. Skinner
  • Biology
    Theoretical and Applied Genetics
  • 2000
Investigation of the stability of the marker fragments through vegetatively, and sexually, propagated plants indicated stable transmission through the sexual phase, and indicated that divergence has resulted in genetically distinct lines persisting in the original region of origin.

Chloroplast microsatellite variations in tetraploid alfalfa

The data presented suggest that cpSSR analysis of tetraploid alfalfa could be used for germplasm polymorphism analysis.

Extent of RFLP variability in tetraploid populations of alfalfa, Medicago sativa

Seven widely-cultivated alfalfa varieties and three ecotypes adapted to Central Italy were used to evaluate the extent of polymorphism in that species and the ability to detect a given fragment in pooled samples was related to the fraction of plants having that fragment among the plants forming the bulk.

Genetic diversity among alfalfa (Medicago sativa L.) cultivars in Northwest China

The RAPD-derived diversity data suggesting that alfalfa cultivars in Northwest China tended to be divergent to adapt to different stress environments are suggested.

AFLP-based assessment of genetic diversity among nine alfalfa germplasms using bulk DNA templates.

The results indicate that use of primer-pair subsets for AFLP analysis of bulk DNA templates could serve as a high-throughput system for accurately characterizing genetic diversity among large numbers of alfalfa populations.

Amplified Fragment Length Polymorphism Marker Diversity among High Yielding Perennial Alfalfa (Medicago sativa L.) Core Collection Accessions

Assessment of amplified fragment length polymorphism (AFLP) marker diversity among 18 high-yielding alfalfa core collection accessions indicated that most hybrids that exhibited positive heterosis and high yield possessed a genetically divergent accession as a parent.

Population-based diallel analyses among nine historically recognized alfalfa germplasms

Heterotic responses were determined by evaluating forage yield of the germplasms and their 36 half-diallel hybrids in seeded plots that were harvested five times in each of 2 years, and results suggest that alfalfa breeders may have capitalized on the heterotic response between Flemish and M. varia during past development of al falfa synthetics adapted to the central and northern latitudes of the United States.

Genetic Diversity and Population Structure of Tetraploid Accessions of the Medicago sativa–falcata Complex

Investigation of the population genetic structure of the tetraploid Medicago sativa–falcata complex revealed that there is a spatial genetic structure among subsp.

Forage Yields of Alfalfa Populations Derived from Parents selected on the Basis of Molecular Marker Diversity

The lack of significant differences between the forage yields of DIS and SIM populations may be due to linkage equilibrium in the population used for selecting parents and the inability to target heterozygosity to specific genome regions affecting yield.