RF cloning: a restriction-free method for inserting target genes into plasmids.

@article{Ent2006RFCA,
  title={RF cloning: a restriction-free method for inserting target genes into plasmids.},
  author={Fusinita M I van den Ent and Jan L{\"o}we},
  journal={Journal of biochemical and biophysical methods},
  year={2006},
  volume={67 1},
  pages={67-74}
}
Restriction-free (RF) cloning provides a simple, universal method to precisely insert a DNA fragment into any desired location within a circular plasmid, independent of restriction sites, ligation, or alterations in either the vector or the gene of interest. The technique uses a PCR fragment encoding a gene of interest as a pair of primers in a linear amplification reaction around a circular plasmid. In contrast to QuickChange site-directed mutagenesis, which introduces single mutations or… CONTINUE READING