REQUIREMENTS FOR EFFICIENT PCR CLAMPING BY LOCKED NUCLEIC ACID OLIGONUCLEOTIES FOR SIMPLE AND SENSITIVE DETECTION OF SOMATIC MUTATIONS

@inproceedings{Shamanin2018REQUIREMENTSFE,
  title={REQUIREMENTS FOR EFFICIENT PCR CLAMPING BY LOCKED NUCLEIC ACID OLIGONUCLEOTIES FOR SIMPLE AND SENSITIVE DETECTION OF SOMATIC MUTATIONS},
  author={Vladimir A. Shamanin and Ilya V. Karpov and Ekaterina E. Pisareva and Nadezhda I. Gutkina and Sergey P. Kovalenko},
  year={2018}
}
pCR clamping/wild-type blocking pCR with non-extendable locked nucleic acid (LNA) oligonucleotides is used for sensitive detection of somatic mutations in tumors. Various versions of the technique use different dNA polymerases and LNA oligonucleotides with and without additional phosphorothioate modifications. Here we Шаманин Владимир александрович, vladimir.shamanin@gmail.com 30 SIBERIAN JoURNAL oF oNCoLoGY. 2018; 17(4): 30–35 31 СИБИРСКИЙ ОНКОЛОГИЧЕСКИЙ ЖУРНАЛ. 2018; 17(4): 30–35… CONTINUE READING

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