REM sleep changes in rats induced by siRNA-mediated orexin knockdown.

  title={REM sleep changes in rats induced by siRNA-mediated orexin knockdown.},
  author={Lichao Chen and Mahesh M Thakkar and Stuart Winston and Yunren Bolortuya and Radhika Basheer and R. W. McCarley},
  journal={The European journal of neuroscience},
  volume={24 7},
Short interfering RNAs (siRNA) targeting prepro-orexin mRNA were microinjected into the rat perifornical hypothalamus. Prepro-orexin siRNA-treated rats had a significant (59%) reduction in prepro-orexin mRNA compared to scrambled siRNA-treated rats 2 days postinjection, whereas prodynorphin mRNA was unaffected. The number of orexin-A-positive neurons on the siRNA-treated side decreased significantly (23%) as compared to the contralateral control (scrambled siRNA-treated) side. Neither the… CONTINUE READING


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