RECEPTOR BINDING AND BIOLOGICAL ACTIVITY OF ANTAGONIST IN A375 HUMAN MELANOMA CELLS+ IL-la, IL-10, IL-lP ANALOGUES AND AN IL-1

@inproceedings{Christen1993RECEPTORBA,
  title={RECEPTOR BINDING AND BIOLOGICAL ACTIVITY OF ANTAGONIST IN A375 HUMAN MELANOMA CELLS+ IL-la, IL-10, IL-lP ANALOGUES AND AN IL-1},
  author={Erica Christen and Gerdien Gamboni and Urda Jess and Jan van Oostrum and Juerg Girard},
  year={1993}
}
A receptor binding assay for IL-1 peptides on human melanoma cells of the A 375 cell line is reported. Strains differing in their sensitivity to the cytotoxic effects of IL-1p were compared. In both strains, binding equilibrium at temperatures between 0" and 37°C was reached after 4 to 8 hours. At 37"C, most of the bound ligand was rapidly internalized leaving a constant level of surface receptors. Scatchard analysis at 0°C revealed a single class of high affinity receptors with a similar KD in… CONTINUE READING