RAPD markers encoding retrotransposable elements are linked to the male sex in Cannabis sativa L.

  title={RAPD markers encoding retrotransposable elements are linked to the male sex in Cannabis sativa L.},
  author={K. Sakamoto and T. Abe and T. Matsuyama and S. Yoshida and N. Ohmido and K. Fukui and S. Satoh},
  volume={48 5},
  • K. Sakamoto, T. Abe, +4 authors S. Satoh
  • Published 2005
  • Medicine, Biology
  • Genome
  • Male-associated DNA sequences were analyzed in Cannabis sativa L. (hemp), a dioecious plant with heteromorphic sex chromosomes. DNA was isolated from male and female plants and subjected to random amplified polymorphic DNA analysis. Of 120 primers, 17 yielded 400 to 1500-bp fragments detectable in male, but not female, plants. These fragments were cloned and used as probes in gel-blot analysis of genomic DNA. When male and female DNA was hybridized with 2 of these male-specific fragments, MADC… CONTINUE READING
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