RAPD markers encoding retrotransposable elements are linked to the male sex in Cannabis sativa L.

  title={RAPD markers encoding retrotransposable elements are linked to the male sex in Cannabis sativa L.},
  author={Koichi Sakamoto and Tomoko Abe and Tomoki Matsuyama and Shigeo Yoshida and Nobuko Ohmido and Kiichi Fukui and Shinobu Satoh},
  volume={48 5},
Male-associated DNA sequences were analyzed in Cannabis sativa L. (hemp), a dioecious plant with heteromorphic sex chromosomes. DNA was isolated from male and female plants and subjected to random amplified polymorphic DNA analysis. Of 120 primers, 17 yielded 400 to 1500-bp fragments detectable in male, but not female, plants. These fragments were cloned and used as probes in gel-blot analysis of genomic DNA. When male and female DNA was hybridized with 2 of these male-specific fragments, MADC… 

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