RAN proteins and RNA foci from antisense transcripts in C9ORF72 ALS and frontotemporal dementia.


The finding that a GGGGCC (G4C2) hexanucleotide repeat expansion in the chromosome 9 ORF 72 (C9ORF72) gene is a common cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) links ALS/FTD to a large group of unstable microsatellite diseases. Previously, we showed that microsatellite expansion mutations can be bidirectionally transcribed and that these mutations express unexpected proteins by a unique mechanism, repeat-associated non-ATG (RAN) translation. In this study, we show that C9ORF72 antisense transcripts are elevated in the brains of C9ORF72 expansion-positive [C9(+)] patients, and antisense GGCCCC (G2C4) repeat-expansion RNAs accumulate in nuclear foci in brain. Additionally, sense and antisense foci accumulate in blood and are potential biomarkers of the disease. Furthermore, we show that RAN translation occurs from both sense and antisense expansion transcripts, resulting in the expression of six RAN proteins (antisense: Pro-Arg, Pro-Ala, Gly-Pro; and sense: Gly-Ala, Gly-Arg, Gly-Pro). These proteins accumulate in cytoplasmic aggregates in affected brain regions, including the frontal and motor cortex, hippocampus, and spinal cord neurons, with some brain regions showing dramatic RAN protein accumulation and clustering. The finding that unique antisense G2C4 RNA foci and three unique antisense RAN proteins accumulate in patient tissues indicates that bidirectional transcription of expanded alleles is a fundamental pathologic feature of C9ORF72 ALS/FTD. Additionally, these findings suggest the need to test therapeutic strategies that target both sense and antisense RNAs and RAN proteins in C9ORF72 ALS/FTD, and to more broadly consider the role of antisense expression and RAN translation across microsatellite expansion diseases.

DOI: 10.1073/pnas.1315438110

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@article{Zu2013RANPA, title={RAN proteins and RNA foci from antisense transcripts in C9ORF72 ALS and frontotemporal dementia.}, author={Tao Zu and Yuanjing Liu and Monica Ba{\~n}ez-Coronel and Tammy Reid and Olga Pletnikova and Jada Lewis and Timothy M. Miller and Matthew B. Harms and Annet E Falchook and Sub H. Subramony and Lyle W. Ostrow and Jeffrey D Rothstein and Juan Troncoso and Laura P. W. Ranum}, journal={Proceedings of the National Academy of Sciences of the United States of America}, year={2013}, volume={110 51}, pages={E4968-77} }