RAISE: a simple and novel method of generating random insertion and deletion mutations

@article{Fujii2006RAISEAS,
  title={RAISE: a simple and novel method of generating random insertion and deletion mutations},
  author={Ryota Fujii and Motomitsu Kitaoka and Kiyoshi Hayashi},
  journal={Nucleic Acids Research},
  year={2006},
  volume={34},
  pages={e30 - e30}
}
Although proteins may be artificially improved by random insertion and deletion mutagenesis methods, these procedures are technically difficult, and the mutations introduced are no more variable than those introduced by the introduction of random point mutations. We describe here a three-step method called RAISE, which is based on gene shuffling and can introduce a wide variety of insertions, deletions and substitutions. To test the efficacy of this method, we used it to mutate TEM beta… CONTINUE READING

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