RAD18 Is a Maternal Limiting Factor Silencing the UV-Dependent DNA Damage Checkpoint in Xenopus Embryos.

@article{Kermi2015RAD18IA,
  title={RAD18 Is a Maternal Limiting Factor Silencing the UV-Dependent DNA Damage Checkpoint in Xenopus Embryos.},
  author={Chames Kermi and Susana Prieto and Siem Van der Laan and Nikolay Tsanov and B{\'e}n{\'e}dicte Recolin and Emmanuelle Uro-Coste and Marie-Bernadette Delisle and Domenico Maiorano},
  journal={Developmental cell},
  year={2015},
  volume={34 3},
  pages={364-72}
}
In early embryos, the DNA damage checkpoint is silent until the midblastula transition (MBT) because of maternal limiting factors of unknown identity. Here we identify the RAD18 ubiquitin ligase as one such factor in Xenopus. We show, in vitro and in vivo, that inactivation of RAD18 function leads to DNA damage-dependent checkpoint activation, monitored by CHK1 phosphorylation. Moreover, we show that the abundance of both RAD18 and PCNA monoubiquitylated (mUb) are developmentally regulated… CONTINUE READING
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