Quiescence Loosens Epigenetic Constraints in Bovine Somatic Cells and Improves Their Reprogramming into Totipotency.

@article{Kallingappa2016QuiescenceLE,
  title={Quiescence Loosens Epigenetic Constraints in Bovine Somatic Cells and Improves Their Reprogramming into Totipotency.},
  author={Prasanna Kumar Kallingappa and Pavla M Turner and Michael P. Eichenlaub and Andria L Green and Fleur C Oback and Alice M. Chibnall and D. N. Wells and Bj{\"o}rn Oback},
  journal={Biology of reproduction},
  year={2016},
  volume={95 1},
  pages={16}
}
Reprogramming by nuclear transfer (NT) cloning forces cells to lose their lineage-specific epigenetic marks and reacquire totipotency. This process often produces molecular anomalies that compromise clone development. We hypothesized that quiescence alters the epigenetic status of somatic NT donor cells and elevates their reprogrammability. To test this idea, we compared chromatin composition and cloning efficiency of serum-starved quiescent (G0) fibroblasts versus nonstarved mitotically… CONTINUE READING
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