QuickStep-Cloning: a sequence-independent, ligation-free method for rapid construction of recombinant plasmids

Abstract

BACKGROUND Molecular cloning is an essential step in biological engineering. Methods involving megaprimer-based PCR of a whole plasmid are promising alternatives to the traditional restriction-ligation-based molecular cloning. Their widespread use, however, is hampered by some of their inherent characteristics, e.g., linear amplification, use of self… (More)
DOI: 10.1186/s13036-015-0010-3

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