Quick recovery and characterization of cell-free DNA in seminal plasma of normozoospermia and azoospermia: implications for non-invasive genetic utilities.

@article{Li2009QuickRA,
  title={Quick recovery and characterization of cell-free DNA in seminal plasma of normozoospermia and azoospermia: implications for non-invasive genetic utilities.},
  author={Hong-gang Li and Shi-Yun Huang and Hui Zhou and Ai-Hua Liao and Cheng-liang Xiong},
  journal={Asian journal of andrology},
  year={2009},
  volume={11 6},
  pages={703-9}
}
We established a quick and reliable method for recovering cell-free seminal DNA (cfsDNA), by using the binding-washing-elution procedure on the DNA purification column. Low variations (below 15%) among the triplicate values of cfsDNA quantity verified the reproducibility of our cfsDNA recovery method. Similar cfsDNA yield and size distribution between seminal plasma acquired by filtration and centrifugation confirmed the presence of cfsDNA. To investigate the general characterization of cfsDNA… CONTINUE READING
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