Quick measurement of protein sulfhydryls with Ellman's reagent and with 4,4′-dithiodipyridine

@article{Riener2002QuickMO,
  title={Quick measurement of protein sulfhydryls with Ellman's reagent and with 4,4′-dithiodipyridine},
  author={Christian K. Riener and Gerald Kada and Hermann J Gruber},
  journal={Analytical and Bioanalytical Chemistry},
  year={2002},
  volume={373},
  pages={266-276}
}
Since its introduction in 1959, Ellman's reagent (5,5′-dithio-bis(2-nitrobenzoic acid)) has been the favorite reagent for spectrophotometric measurement of protein sulfhydryls. Meanwhile however, evidence has accumulated that many protein sulfhydryls give an incomplete reaction with Ellman's reagent, even during prolonged assay times. In the present study, the kinetic problem was solved by including cystamine as a "mediator" between the protein sulfhydryl and Ellman's reagent, as previously… CONTINUE READING

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