Quick histochemical staining method for measuring lactate dehydrogenase C4 activity in human spermatozoa.

@article{Cui2015QuickHS,
  title={Quick histochemical staining method for measuring lactate dehydrogenase C4 activity in human spermatozoa.},
  author={Zhaolei Cui and Liangyuan Chen and Yaohua Liu and Zhangxin Zeng and Fenghua Lan},
  journal={Acta histochemica},
  year={2015},
  volume={117 3},
  pages={
          235-42
        }
}
The enzyme activity of lactate dehydrogenase C4 (LDH-C4, due to tetrameric nature of C-subunit) has been proposed as an important parameter in evaluating sperm motility and semen quality. A novel histochemical staining method for detecting LDH-C4 activity in human spermatozoa is described in this report. The staining working solution comprises sodium 2-hydroxybutyrate (an affinity substrate of LDH-C4), nitrotetrazolium blue chloride (NBT), nicotinamide adenine dinucleotide (NAD) and naphthol… Expand
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