Quick-freeze/deep-etch visualization of age-related lipid accumulation in Bruch's membrane.


PURPOSE To examine age-related changes in the ultrastructure of Bruch's membrane with quick-freeze/deep-etch (QFDE) and conventional thin-section transmission electron microscopy (TEM). METHODS Four eyes from human donors aged 27, 41, 76, and 78 years were preserved within 4 hours of death. Full-thickness tissue blocks from the macula were prepared for… (More)


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