Quercetin production from rutin by a thermostable β-rutinosidase from Pyrococcus furiosus

@article{Nam2011QuercetinPF,
  title={Quercetin production from rutin by a thermostable β-rutinosidase from Pyrococcus furiosus},
  author={Hyun-Koo Nam and Seung-Hye Hong and Kyung-Chul Shin and Deok-Kun Oh},
  journal={Biotechnology Letters},
  year={2011},
  volume={34},
  pages={483-489}
}
Pyrococcus furiosus β-glucosidase converted rutin to quercetin and rutinose disaccharide with a ratio of 1:1, with no glucose, l-rhamnose, and isoquercitrin, indicating that the enzyme is a β-rutinosidase. The specific activity for flavonoid glycosides followed the order of isoquercitrin > quercitrin > rutin. The conversion of rutin to quercetin was optimal at pH 5.0 and 95°C in the presence of 0.5% dimethyl sulfoxide with a half-life of 101 h, a k cat of 1.6 min−1, and a K m of 0.3 mM. Under… CONTINUE READING