Quenching of tryptophan fluorescence by brominated phospholipid.

  title={Quenching of tryptophan fluorescence by brominated phospholipid.},
  author={E J Bolen and Peter Holloway},
  volume={29 41},
Bromolipids [1-palmitoyl-2-(dibromostearoyl)phosphatidylcholine] with bromines at the 4,5-, 6,7-, 9,10-, 11,12-, and 15,16-positions were used to examine the fluorescence quenching of a synthetic, membrane-spanning peptide (Lys2-Gly-Leu8-Trp-Leu8-Lys-Ala-amide) incorporated into both small and large unilamellar vesicles. The peptide-lipid vesicles were analyzed to show that at least 75% of the peptide was in a transbilayer configuration, placing the single tryptophan in its predicted place in… CONTINUE READING