Quenching of the tyrosyl and tryptophyl fluorescence of subtilisins Carlsberg and Novo by iodide.

@article{Brown1977QuenchingOT,
  title={Quenching of the tyrosyl and tryptophyl fluorescence of subtilisins Carlsberg and Novo by iodide.},
  author={Michael F Brown and Siti Muhayyah Omar and R A Raubach and Thomas J. Schleich},
  journal={Biochemistry},
  year={1977},
  volume={16 5},
  pages={
          987-92
        }
}
The tyrosyl and tryptophyl fluorescence of diisopropylphosphorylsubtilisins Carlsberg and Novo, respectively, is quenched efficiently by I- but is not significantly affected by Cs+. The I-quenching data were analyzed using a modified Stern-Volmer treatment (Lehrer, S.S. (1971), Biochemistry 10, 3254), yielding values for the effective fraction of accessible protein fluorescence of 90-95 and 88-92% for the tyrosyl and tryptophyl emission of diisopropyl-phosphorylsubtilising Carlsberg and Novo… CONTINUE READING
BETA

References

Publications referenced by this paper.

arrived at similar conclusions from circular polarization of luminescence measurements

  • Schlessinger
  • 1975
1 Excerpt

Similar Papers

Loading similar papers…