Quenching of fluorescent nucleotides bound to myosin: a probe of the active-site conformation.

Abstract

The conformation of the active ATPase site of myosin subfragment 1 (S1) and actomyosin in myofibrils was probed by measuring the solvent accessibility of the bound ethenonucleotides epsilon ADP and epsilon ATP (during steady-state hydrolysis). Solvent accessibility was determined by measuring the quenching of fluorescence produced by the solvent-phase… (More)

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Cite this paper

@article{FranksSkiba1994QuenchingOF, title={Quenching of fluorescent nucleotides bound to myosin: a probe of the active-site conformation.}, author={Kathleen E Franks-Skiba and T. Hwang and Richard Cooke}, journal={Biochemistry}, year={1994}, volume={33 42}, pages={12720-8} }