Quenching by acrylamide and temperature of a fluorescent probe attached to the active site of Ribonuclease

Abstract

The fluorescence properties of ribonuclease labelled at its active site with N-(iodoacetylamino)-ethyl-5-naphthylamine-1-sulfonic acid have been studied at different temperatures and in the presence of acrylamide. The rate constant for the quenching of the fluorescence of labelled ribonuclease by acrylamide is apparently not limited by the “accessibility… (More)
DOI: 10.1007/BF00542558

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@article{Jullien1986QuenchingBA, title={Quenching by acrylamide and temperature of a fluorescent probe attached to the active site of Ribonuclease}, author={Marc Jullien and J. -R. Garel and Fabienne M{\'e}rola and J. -C. Brochon}, journal={European Biophysics Journal}, year={1986}, volume={13}, pages={131-137} }