Quaternary structure of Dioclea grandiflora lectin assessed by equilibrium sedimentation and crystallographic analysis of recombinant mutants.

@article{ZamoraCaballero2015QuaternarySO,
  title={Quaternary structure of Dioclea grandiflora lectin assessed by equilibrium sedimentation and crystallographic analysis of recombinant mutants.},
  author={Sara Zamora-Caballero and Alicia As{\'i}n P{\'e}rez and L Puig Sanz and Jer{\'o}nimo Bravo and Juan J Calvete},
  journal={FEBS letters},
  year={2015},
  volume={589 18},
  pages={2290-6}
}
The structural basis of the pH dependency of the dimer-tetramer transition exhibited by Brinda's type II Diocleinae lectins was investigated by equilibrium sedimentation and X-ray crystal structure determination of recombinant wild-type and site-directed single and double mutants of the pH-stable tetrameric Dioclea grandiflora lectin (r-αDGL). Releasing the peripheral site interdimeric contact between R60 and D78 rendered a mutant displaying dimer-tetramer equilibrium in the pH range equivalent… CONTINUE READING

From This Paper

Topics from this paper.
1 Citations
0 References
Similar Papers

Similar Papers

Loading similar papers…