Quaternary re-arrangement analysed by spectral enhancement: the interaction of a sporulation repressor with its antagonist.

@article{Scott1999QuaternaryRA,
  title={Quaternary re-arrangement analysed by spectral enhancement: the interaction of a sporulation repressor with its antagonist.},
  author={David J. Scott and S Leejeerajumnean and James A Brannigan and Richard J. Lewis and Anthony James Wilkinson and James G Hoggett},
  journal={Journal of molecular biology},
  year={1999},
  volume={293 5},
  pages={997-1004}
}
The protein/protein interaction between SinI and SinR has been studied by analytical ultracentrifugation and gel electrophoresis in an attempt to understand how these proteins contribute to developmental control of sporulation in Bacillus subtilis. SinR was found to be tetrameric, while SinI was found to exist as monomers and dimers in a rapidly reversible equilibrium. Labelling of SinR by incorporating the tryptophan analogue 7-azatryptophan (7AW) into the protein in place of tryptophan shifts… CONTINUE READING

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