Quaternary and quinternary structures of native chromatin DNA in liver nuclei: differential scanning calorimetry.

Abstract

Differential scanning calorimetry of chromatin isolated from rat liver cells revealed three discrete thermal transitions whose temperatures and melting enthalpies depend on ionic strength in the range 0 to 600 millimolar NaCl. Intact nuclei showed a fourth thermal transition at a lower temperature and different melting enthalpies for the other three… (More)

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