Quantitative two-photon flow cytometry--in vitro and in vivo.

  title={Quantitative two-photon flow cytometry--in vitro and in vivo.},
  author={Cheng Frank Zhong and Eric R. Tkaczyk and Thommey P. Thomas and Jing Yong Ye and Andrzej Myc and Anna U. Bielinska and Zhengyi Cao and Istv{\'a}n J Majoros and Balazs Keszler and James R Baker and Theodore B Norris},
  journal={Journal of biomedical optics},
  volume={13 3},
Flow cytometry is a powerful technique for quantitative characterization of fluorescence in cells. Quantitation is achieved by ensuring a high degree of uniformity in the optical excitation and detection, generally by using a highly controlled flow. Two-photon excitation has the advantages that it enables simultaneous excitation of multiple dyes and achieves a very high SNR through simplified filtering and fluorescence background reduction. We demonstrate that two-photon excitation in… CONTINUE READING