Quantitative thin-layer chromatography of pyrimethamine and related diaminopyrimidines in body fluids and tissues.

  title={Quantitative thin-layer chromatography of pyrimethamine and related diaminopyrimidines in body fluids and tissues.},
  author={R. Deangelis and W. S. Simmons and C. A. Nichol},
  journal={Journal of chromatography},
  volume={106 1},
Several 2,4-diaminopyrimidines which inhibit the enzyme dihydrofolate reductase are quantitated following extraction and separation on silica gel thin-layer chromatographic plates. These compounds are candidates for the treatment of brain tumors and meningeal leukemia, because they have the ability to cross the blood-brain barrier. The ultraviolet absorption of the pyrimidine ring at 275 nm is utilized to quantitate these compounds on thin-layer chromatographic plates with a scanning instrument… Expand
Simultaneous determination of pyrimethamine and mefloquine in human plasma by high-performance liquid chromatography with ultraviolet detection.
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  • C. Midskov
  • Chemistry, Medicine
  • Journal of chromatography
  • 1984
A sensitive and selective reversed-phase high-performance liquid chromatographic assay has been developed and proved convenient for pharmacokinetic studies in man following oral co-administration of pyrimethamine and sulfadoxine. Expand
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A high-pressure liquid chromatographic assay for metoprine levels in aqueous media, serum, and tissue extracts has been developed that can measure concentrations of the drug in the 25-100 ng rangeExpand
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High Performance Liquid Chromatographic Assay with Fluorescence Detection for the Analysis of 1954U89, 1,3-Diamino-7-(1-ethylpropyl)-8-methyl-7H-pyrrolo-(3,2-f)-quinazoline, in Plasma
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A simple spectrodensitometric method for the direct determination of trimethoprim and sulfamethoxazole is based upon measurement of the absorbance of the two compounds on silica gel plates irradiatedExpand
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Treatment of meningeal leukemia with pyrimethamine.
Considerations regarding lipid solubility, body distribution, and easily reversible toxicity of pyrimethamine suggest that further therapeutic trials are warranted, especially in childhood lymphoblastic leukemia. Expand