Quantitative thin-layer chromatography of pyrimethamine and related diaminopyrimidines in body fluids and tissues.

@article{Deangelis1975QuantitativeTC,
  title={Quantitative thin-layer chromatography of pyrimethamine and related diaminopyrimidines in body fluids and tissues.},
  author={R. Deangelis and W. S. Simmons and C. A. Nichol},
  journal={Journal of chromatography},
  year={1975},
  volume={106 1},
  pages={
          41-9
        }
}
Several 2,4-diaminopyrimidines which inhibit the enzyme dihydrofolate reductase are quantitated following extraction and separation on silica gel thin-layer chromatographic plates. These compounds are candidates for the treatment of brain tumors and meningeal leukemia, because they have the ability to cross the blood-brain barrier. The ultraviolet absorption of the pyrimidine ring at 275 nm is utilized to quantitate these compounds on thin-layer chromatographic plates with a scanning instrument… Expand
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