Quantitative testing of the methodology for genome size estimation in plants using flow cytometry: a case study of the Primulina genus

@inproceedings{Wang2015QuantitativeTO,
  title={Quantitative testing of the methodology for genome size estimation in plants using flow cytometry: a case study of the Primulina genus},
  author={Jing Wang and Juan Liu and Ming Kang},
  booktitle={Front. Plant Sci.},
  year={2015}
}
Flow cytometry (FCM) is a commonly used method for estimating genome size in many organisms. The use of FCM in plants is influenced by endogenous fluorescence inhibitors and may cause an inaccurate estimation of genome size; thus, falsifying the relationship between genome size and phenotypic traits/ecological performance. Quantitative optimization of FCM methodology minimizes such errors, yet there are few studies detailing this methodology. We selected the genus Primulina, one of the most… CONTINUE READING
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