Quantitative telomeric overhang determination using a double-strand specific nuclease

@inproceedings{Zhao2008QuantitativeTO,
  title={Quantitative telomeric overhang determination using a double-strand specific nuclease},
  author={Yong Zhao and Hirotoshi Hoshiyama and Jerry W Shay and Woodring E Wright},
  booktitle={Nucleic acids research},
  year={2008}
}
Telomeres terminate in 3' overhangs that function in end protection and the formation of t-loops. Determining the steps and factors involved in overhang processing is compromised by the inability to easily and accurately determine overhang size in the presence of many kilobases of double-stranded telomeric DNA. We here describe the use of a double-strand specific nuclease (DSN) that entirely digests double-stranded DNA including telomeres, leaving the overhangs intact so that they can be… CONTINUE READING

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