Quantitative specificity of the Mnt repressor.

  title={Quantitative specificity of the Mnt repressor.},
  author={D. S. Fields and Y He and A Al-Uzri and Gary D. Stormo},
  journal={Journal of molecular biology},
  volume={271 2},
The Mnt protein of Salmonella phage P22 binds site-specifically to its operator. To better understand this binding we used dideoxy DNA sequencing in a quantitative manner to determine the relative binding constants, and hence the relative free energies, of wild-type Mnt protein to a substantial number of variants of its operator. These measurements were supported by experiments which used the SELEX procedure to generate a set of operators from an initially randomized population. In the… CONTINUE READING
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