Quantitative single molecule measurements on the interaction forces of poly(L-glutamic acid) with calcite crystals.

Abstract

The interaction between poly(L-glutamic acid) (PLE) and calcite crystals was studied with AFM-based single molecule force spectroscopy. Block copolymers of poly(ethylene oxide) (PEO) and PLE were synthesized and covalently attached to the tip of an AFM cantilever. In desorption measurements the molecules were allowed to adsorb on the calcite crystal faces and afterward successively desorbed. The corresponding desorption forces were detected with high precision, showing for example a force transition between the two blocks. Because of its importance in the crystallization process in biominerals, the PLE-calcite interaction was investigated as a function of the pH as well as the calcium concentration of the aqueous solution. The sensitivity of the technique was underlined by resolving different interaction forces for calcite (104) and calcite (100).

Cite this paper

@article{Sonnenberg2007QuantitativeSM, title={Quantitative single molecule measurements on the interaction forces of poly(L-glutamic acid) with calcite crystals.}, author={Lars Sonnenberg and Yufei Luo and Helmut Schlaad and Markus Seitz and Helmut C{\"{o}lfen and Hermann Eduard Gaub}, journal={Journal of the American Chemical Society}, year={2007}, volume={129 49}, pages={15364-71} }