Quantitative reverse transcription-polymerase chain reaction detection of cytokeratin 20 in noncolorectal lymph nodes.

@article{Soong2001QuantitativeRT,
  title={Quantitative reverse transcription-polymerase chain reaction detection of cytokeratin 20 in noncolorectal lymph nodes.},
  author={Richie Soong and Kurt H Beyser and Oliver Basten and Andreas Kalbe and Josef Rueschoff and Karim Tabiti},
  journal={Clinical cancer research : an official journal of the American Association for Cancer Research},
  year={2001},
  volume={7 11},
  pages={3423-9}
}
PURPOSE Unexpected reverse transcription-PCR detection of cytokeratin 20 (CK20) in samples from healthy individuals and cancer types not expected to express CK20 has cast uncertainty on the role of CK20 as a specific marker of disseminated colorectal cells. We aimed to clarify the specificity of CK20 by examining its expression profile by real-time reverse transcription-PCR. EXPERIMENTAL DESIGN A quantitative real-time PCR assay on the LightCycler instrument was developed and used to examine… CONTINUE READING
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The human gene encoding 3428 CK20 Quantification in Lymph Nodes Research

  • R. Moll, R. Zimbelmann, +5 authors W. W. Franke
  • on April
  • 2001

Quantitative analysis of cytokeratin 20 gene expression using RT-PCR and capillary electrophoresis with fluorescent DNA detection

  • J. A. van Eekelen, F. V. Shammas, L. Wee, R. Heikkila, A. Osland
  • Clin. Biochem.,
  • 2000

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