Quantitative reverse transcription-polymerase chain reaction detection of cytokeratin 20 in noncolorectal lymph nodes.

@article{Soong2001QuantitativeRT,
  title={Quantitative reverse transcription-polymerase chain reaction detection of cytokeratin 20 in noncolorectal lymph nodes.},
  author={Richie Soong and Kurt H Beyser and Oliver Basten and Andreas Kalbe and Josef Rueschoff and Karim Tabiti},
  journal={Clinical cancer research : an official journal of the American Association for Cancer Research},
  year={2001},
  volume={7 11},
  pages={3423-9}
}
PURPOSE Unexpected reverse transcription-PCR detection of cytokeratin 20 (CK20) in samples from healthy individuals and cancer types not expected to express CK20 has cast uncertainty on the role of CK20 as a specific marker of disseminated colorectal cells. We aimed to clarify the specificity of CK20 by examining its expression profile by real-time reverse transcription-PCR. EXPERIMENTAL DESIGN A quantitative real-time PCR assay on the LightCycler instrument was developed and used to examine… CONTINUE READING