Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods.

@article{Schmittgen2000QuantitativeRT,
  title={Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods.},
  author={Thomas D. Schmittgen and Brian A Zakrajsek and A G Mills and Vladimir V. Gorn and M J Singer and Michael W. Reed},
  journal={Analytical biochemistry},
  year={2000},
  volume={285 2},
  pages={
          194-204
        }
}
Four quantitative reverse transcription-PCR (RT-PCR) methods were compared to evaluate the time course of mRNA formation and decay. Mouse fibroblasts (NIH 3T3) transfected with the human beta-globin open reading frame/c-myc 3'-untranslated region chimeric gene under control of the c-fos promoter (fos-glo-myc) were used for serum-inducible transcription. The amount of fos-glo-myc mRNA, relative to beta-actin, was measured by quantitative, RT-PCR at various times following the addition of serum… Expand
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