Quantitative reverse transcription-PCR comparison of tumor cell enrichment methods.

@article{Ladnyi2001QuantitativeRT,
  title={Quantitative reverse transcription-PCR comparison of tumor cell enrichment methods.},
  author={Andr{\'a}s Lad{\'a}nyi and Richie Soong and Karim Dr. Tabiti and B. J. Molnar and Zsolt Tulassay},
  journal={Clinical chemistry},
  year={2001},
  volume={47 10},
  pages={
          1860-3
        }
}
The feasibility of detecting disseminated tumor cells through reverse transcription-PCR (RT-PCR) detection of tissue-specific genetic markers in extra-tumor compartments is currently unclear because of conflicting findings on assay sensitivity and specificity between studies [for reviews, see Refs. (1)(3)]. One potential source of these assay discrepancies includes the different sample preparations and analysis methods used between laboratories (4). Many studies have used nonenriched (NE) whole… CONTINUE READING

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