Quantitative reverse transcription-PCR assay of the RNA component of human telomerase using the TaqMan fluorogenic detection system.

@article{Yajima1998QuantitativeRT,
  title={Quantitative reverse transcription-PCR assay of the RNA component of human telomerase using the TaqMan fluorogenic detection system.},
  author={Tomomi Yajima and Atsuhito Yagihashi and Hidekazu Kameshima and Daisuke Kobayashi and Daisuke Furuya and Koichi Hirata and Naoki Watanabe},
  journal={Clinical chemistry},
  year={1998},
  volume={44 12},
  pages={2441-5}
}
We established the validity of a quantitative reverse transcription (RT)-PCR assay for the RNA component of human telomerase (hTR), using the TaqMan fluorogenic detection system. Using this assay, we quantified hTR expression in two human pancreatic cancer cell lines, ASPC-1 and MIAPaCa-2. Our results indicated that hTR expression in MIAPaCa-2 was 1.99-fold higher than that in ASPC-1 cells. This TaqMan RT-PCR assay appears to be useful in determining the amount of hTR in clinical specimens. 
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