Quantitative real-time RT-PCR detection of breast cancer micrometastasis using a multigene marker panel.

@article{Mitas2001QuantitativeRR,
  title={Quantitative real-time RT-PCR detection of breast cancer micrometastasis using a multigene marker panel.},
  author={Michael C Mitas and Kaidi Mikhitarian and Christian Walters and Paul L. Baron and Bruce M. Elliott and Thomas E Brothers and Jacob G. Robison and John S. Metcalf and Yuko Y. Palesch and Zhengdong Zhang and William E. Gillanders and David John Cole},
  journal={International journal of cancer},
  year={2001},
  volume={93 2},
  pages={
          162-71
        }
}
Real-time RT-PCR is a relatively new technology that uses an online fluorescence detection system to determine gene expression levels. It has the potential to significantly improve detection of breast cancer metastasis by virtue of its exquisite sensitivity, high throughput capacity and quantitative readout system. To assess the utility of this technology in breast cancer staging, we determined the relative expression levels of 12 cancer-associated genes (mam, PIP, mamB, CEA, CK19, VEGF, erbB2… CONTINUE READING
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